Column: BioCore HIC-Butyl, 5 μm
Dimension: 4.6×100 mm
Mobile Phase: A) 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0
B) 100 mM phosphate buffer, pH7.0
C) Isopropanol
Gradient: t(min) %A %B %C
0 60 40 0
20 0 80 20
25 0 80 20
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 214 nm
Sample: 双抗