Column: BioCore RP-Butyl, 5 μm
Dimension: 3.0×50 mm
Mobile Phase: A) 0.1% HCOOH in H2O
B) 0.1% HCOOH in MeCN
Gradient: t(min) %A %B
0 95 5
1 95 5
1.1 95 5
1.2 5 95
4 5 95
4.1 95 5
7 95 5
Flow Rate: 0.6 mL/min
Temperature: 60 °C
Injection: ~1 μg
Detection: QE
Sample: Monoclonal Antibody