Column: BioCore SAX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM Tris, pH8.5
B) 500 mM NaCl in 20 mM Tris, pH8.5
Gradient: t(min) %A %B
-15 100 0
0 100 0
15 0 100
15.1 0 100
20 0 100
Flow Rate: 1.0 mL/min
Injection: 10 μL
Temperature: 30 °C
Detection: UV 280 nm
Sample: BSA (5.0 mg/mL in H2O)