融合蛋白在BioCore SCX色谱柱上的分离（离子交换）

Column: BioCore SCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM MES, pH6.0
B) 300 mM NaCl in 20 mM MES, pH6.0
Gradient: t (min) %A %B
-15 72 28
0 72 28
25 45 55
25.1 0 100
28 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: 融合蛋白 (2.5 mg/mL in Mobile Phase A)