Columns: ChromCore C18, 5 μm
ChromCore 120 C18, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 0.01 M磷酸氢二钾缓冲液, pH8.5
B) 90/10 v/v乙腈/四氢呋喃
Gradient: t (min) %A %B
0 95 5
3 92 8
10 90 10
14 85 15
16 78 22
30 61 39
35 61 39
40 95 5
50 95 5
Flow Rate: 1.0 mL/min
Temperature: 40 °C
Injection: 5 μL
Detection: UV 300 nm
Sample: 磷酸特地唑胺
Peaks: 1~3. 杂质
4. 特地唑胺
5~11. 杂质