Column: BioCore Protein A, 15 μm
Dimension: 2.1×30 mm
Mobile Phase: 150 mM NaCl in 50 mM PBS, pH7.0
150 mM NaCl in 50 mM PBS, pH2.5
Gradient: t (min) %A %B
0 100 0
0.5 100 0
0.51 0 100
1.5 0 100
1.51 100 0
2.5 100 0
Flow Rate: 2.0 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 280 nm
Sample: IgG (2.4 mg/mL)
Peaks: 1. Flowthrough
2. IgG