Column: BioCore WCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM MES, pH6.5
B) 300 mM NaCl in 20 mM MES, pH6.5
Gradient: t (min) %A %B
-15 78 22
0 78 22
0.1 78 22
20 63 37
20.1 0 100
23 0 100
Flow Rate: 0.6 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: Rituximab biosimilar injection solution (5 mg/mL in water)