抗体在BioCore Protein A色谱柱上的分离（效价分析）

Column: BioCore Protein A, 15 μm
Dimension: 2.1×50 mm
Mobile Phase: A) 150 mM NaCl in 50 mM phosphate buffer, pH7.0
B) 150 mM NaCl in 50 mM phosphate buffer, pH2.5
Gradient: t (min) %A %B
0 100 0
1 100 0
1.01 0 100
2.2 0 100
2.21 100 0
4 100 0
Flow Rate: 2.0 mL/min
Temperature: 25 °C
Injection: 20 μL
Detection: UV 280 nm
Sample: 抗体