单抗在BioCore SEC-300/SEC-500色谱柱上的分离

Columns: BioCore SEC-300, 5 μm
BioCore SEC-500, 5 μm
Dimension: 7.8×300 mm
Mobile Phase: 0.3 M NaCl in 50 mM phosphate buffer, pH6.8
Flow Rate: 0.7 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 214 nm
Sample: 单抗
Peaks: 1. 多聚 (900KD)
2. 单体 (150KD)
3. 重链 (50KD)
4. 轻链 (25KD)