Column: BioCore HIC-Butyl, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 1 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0
B) 100 mM phosphate buffer, pH7.0
Gradient: t (min) %A %B
0 100 0
60 0 100
Flow Rate: 0.5 mL/min
Temperature: 25 °C
Injection: 10 μL
Detection: UV 280 nm