标准蛋白在BioCore HIC-Butyl色谱柱上的分离（疏水作用）

Column: BioCore HIC-Butyl, 5 μm
Dimension: 4.6×100 mm
Mobile Phase: A) 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0
B) 100 mM phosphate buffer, pH7.0
Gradient: t (min) %A %B
-10 100 0
0 100 0
1 100 0
15 0 100
20 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Peaks: (~1 mg/mL each in Mobile Phase A)
1. Ribonuclease A
2. Myoglobin
3. Lysozyme
4. α-Chymotrypsinogen A