Column: DNACore 1000 C18, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 0.1 M CH3COOH-Et3N solution, pH7.0
B) 25/75 v/v MeCN/ 0.1 M CH3COOH-Et3N solution, pH7.0
Gradient: t (min) %A %B
0 60 40
30 35 65
31 60 40
40 60 40
Flow Rate: 1.0 mL/min
Temperature: 60 °C
Injection: 10 μL
Detection: UV 254 nm
Sample: 疫苗中的RNA长链 (1000~2000 nt)