Column: BioCore SEC-300, 3 μm
Dimension: 4.6×300 mm
Mobile Phase: 90/10 v/v 300 mM NaCl in 50 mM phosphate
buffer, pH6.8/MeCN
Flow Rate: 0.21 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 280 nm
Sample: mAb size variant standard (1.0 mg/mL)