Columns: BioCore SEC-150, 5 μm
BioCore SEC-300, 5 μm
BioCore SEC-500, 5 μm
Dimension: 4.6×300 mm
Mobile Phase: 150 mM phosphate buffer, pH6.8
Flow Rate: 0.35 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 280 nm
Sample: IgG4 (16 mg/mL in water)
Peaks: 1. IgG4 Aggregates
2. IgG4