Column: BioCore SEC-300, 5 μm
Dimension: 7.8×300 mm
Mobile Phase: 90/10 v/v 300 mM NaCl in 50 mM phosphate buffer,
pH6.8/MeCN
Flow Rate: 0.6 mL/min
Temperature: 30 °C
Injection: 20 μL
Detection: UV 280 nm
Sample: IgG4 (~1.5 mg/mL in H2O)