Column: BioCore WCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM MES, pH6.5
B) 300 mM NaCl in 20 mM MES, pH6.5
Gradient: t (min) %A %B
-15 72 28
0 72 28
0.1 72 28
20 57 43
20.1 0 100
23 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 25 μL
Detection: UV 280 nm
Sample: IgG2 (1 mg/mL in water)