Column: BioCore WCX, 10 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 20 mM MES, pH6.5
B) 150 mM NaCl in 20 mM MES, pH6.5
Gradient: t (min) %A %B
-15 95 5
0 95 5
0.1 95 5
40 80 20
40.1 0 100
43 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: IgG1 (~2.5 mg/mL in Mobile Phase A)