Column: BioCore Glycan, 3 μm
Dimension: 3.0×100 mm
Mobile Phase: A) 50 mM CH3COONH4, pH4.4
B) MeCN
Gradient: t(min) %A %B
0 25 75
2 25 75
37 46 54
37.1 25 75
40 25 75
Flow Rate: 0.4 mL/min
Temperature: 65 °C
Injection: 30 μL
Detection: Ex/Em= 250 nm/428 nm
Sample: N-Glycans of Avastin