Platinum® Pfx DNA 聚合酶

Platinum® Pfx DNA 聚合酶

极高保真度的热稳定 PCR 酶

1.保真度较 Taq DNA 聚合酶高 26 倍
2.扩增片段长达 12 kb
3.在室温下建立反应体系

Platinum® Pfx DNA 聚合酶 是高保真 PCR 应用中 DNA 片段扩增的理想选择。高保真度由专有的酶制剂提供，该酶制剂含有来自热球菌属KOD的重组DNA聚合酶，具有校对（3'→5'核酸外切酶）活性。Platinum®类抗体技术提供了一种简单、自动、热启动的方法，可提高 PCR 特异性（见图）。PCRx 增强剂解决方案可实现更高的引物特异性，更广泛的镁浓度余量，更广泛的退火温度参数，并可改善Platinum® Pfx DNA聚合酶的热稳定性。PCRx 增强剂解决方案还有助于优化有问题和/或富含 GC 的模板的 PCR。Platinum® Pfx DNA 聚合酶提供：

Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates. Platinum® Pfx DNA Polymerase provides:

1.保真度较 Taq DNA 聚合酶高 26 倍
2.扩增片段长达 12 kb
3.在室温下建立反应体系

从复杂的基因组、病毒和质粒模板中扩增 DNA;和 RT-PCR。Platinum® Pfx DNA 聚合酶在需要非常高的保真度时特别有效。

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assemblyApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum® Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

单元定义
一个单元指：在74°C下30分钟内将10毫摩尔脱氧核糖核苷酸掺入酸性可沉淀材料中。

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C.

Platinum® Pfx DNA 聚合酶信息由 赛默飞世尔科技生命科学产品为您提供，如您想了解更多关于Platinum® Pfx DNA 聚合酶报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途