产地：MBI 

货号：ER0891

Conditions for 100% Activity

1X Buffer G:

10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

Bsh1285I is supplied in:

10 mM Tris-HCl (pH 7.4 at 25°C), 150 mM KCl, 1 mM DTT, 1 mM EDTA, 0.15% Triton X-100, 0.5 mg ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 50-fold overdigestion with Bsh1285I, more than 95% of the DNA fragments can be ligated and recut.