产地：MBI 

货号：ER1001

Conditions for 100% Activity

1X Buffer O:

50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

BshNI is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Digestion of Agarose-embedded DNA

Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Ligation and Recleavage

After 50-fold overdigestion with BshNI, more than 95% of the DNA fragments can be ligated and recut.