产地：MBI 

货号：ER1101

Conditions for 100% Activity

1X Buffer B:

10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.

Incubate at 37°C.

Ligation and Recleavage

After 50-fold overdigestion with HphI, more than 70% of the DNA fragments can be ligated and more than 90% of these can be recut.

Storage Buffer

HphI is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

ER1101	300 u (10 u/µl)	1.00 ml	1.00 ml	ER1101
ER1102	1500 u (10 u/µl)	1.00 ml	1.00 ml	ER1102