ER1141	400 u (10 u/µl)	1.00 ml	1.00 ml	ER1141
ER1142	2000 u (10 u/µl)	1.00 ml	1.00 ml	ER1142

产地：MBI 

货号：ER1142

Conditions for 100% Activity

1X Buffer R:

10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.

Incubate at 65°C. To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil.

Ligation and Recleavage

After 50-fold overdigestion with TaiI, more than 95% of DNA fragments can be ligated and recut.

Storage Buffer

TaiI is supplied in:

10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.