产地：MBI
货号：ER1191 Conditions for 100% Activity
1X Buffer SdaI:
37 mM Tris-acetate (pH 7.0 at 37°C), 15 mM Mg-acetate, 150 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
SdaI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 5-fold overdigestion with SdaI, more than 95% of the DNA fragments can be ligated and recut.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
ER1191 |
300 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1191 |
|
ER1192 |
1500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1192 |
|
