产地：MBI 
货号：ER2011
Storage Buffer
Nt.Bpu10I is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA, 50% glycerol.
Production of single-stranded circular DNA from supercoiled double-stranded plasmids in vitro with subsequent use in DNA sequencing, site-specific mutagenesis, etc.
Creation of nested deletions.
Vector preparation for ligation independent cloning method.
Preparations of covalently closed, double- stranded linear DNA molecules.
Conditions for 100% Activity
1X Buffer R:
10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl, 0.1 mg/ml BSA.
Incubate at 37°C.