SRT体积排阻色谱柱-分析测试百科网

SRT体积排阻色谱柱

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品牌：美国赛分
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产地类别：进口
产地：-
货期：现货
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概述 Sepax SEC键合固定相是采用专利的表面修饰技术，通过在高纯度具有良好机械稳定性的硅胶基质上，键合一层均匀的纳米厚度的中性亲水涂层而制备得到。由于采用可控的化学修饰技术，因此能确保柱与柱之间有着可靠的重现性。SEC填料采用化学键合

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耗材描述

概述
Sepax SEC键合固定相是采用专利的表面修饰技术，通过在高纯度具有良好机械稳定性的硅胶基质上，键合一层均匀的纳米厚度的中性亲水涂层而制备得到。由于采用可控的化学修饰技术，因此能确保柱与柱之间有着可靠的重现性。SEC填料采用化学键合技术，表面亲水涂层覆盖完全，因此不仅具有优异的稳定性，而且对蛋白等生物样品的非特异性吸附作用也非常小。精心设计的大孔体积可保证高的分离容量以及优异的分辨率。广泛应用于生物分子及水溶性聚合物的分离和检测。

SRT体积排阻色谱柱优点

大的孔体积，高的柱容量，高的柱效，良好的重现性
宽的pH范围（pH范围2-8.5）
寿命长，一般在700-1000针
低盐浓度洗脱，适合LC-MS分析
对于生物样品常见的PEG或者吐温80等物质无强吸附
高的蛋白回收率，且蛋白不易失活
极低的非特异性吸附
不会出现二聚体与单体出峰紊乱的现象
解决多维色谱分析应用

应用领域

蛋白药物（人血白蛋白、免疫球蛋白、HGH、EPO等）
多肽(Peptide)
水溶性多聚物（多糖、PEG）
抗生素（头孢类抗生素）
抗体（Antibodies）、疫苗（Vaccine）
病毒蛋白(Virus Protein)、细胞发酵液、DNA

技术参数

固定相	SRT SEC-100	SRT SEC-150	SRT SEC-300	SRT SEC-500	SRT SEC-1000	SRT SEC-2000
材料	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica
颗粒大小	5 mm	5 mm	5 mm	5 mm	5 mm	5 mm
孔径 (Å)	~ 100	~ 150	~ 300	~ 500	~ 1,000	~ 2,000
蛋白分子量范围	100 - 100,000	500 - 150,000	5,000 – 1,250,000	15,000 - 5,000,000	50,000 - 7,500,000	> 10,000,000
水溶性聚合物分子量范围	500-10,000	500-25,000	1,000-100,000	2,500-500,000	5,000-1,500,000	50,000->2,500,000
pH稳定性	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
反压 (psi for a 7.8x300 mm)	~ 700	~ 700	~ 700	~700	~700	~700
最大压力 (psi)	~ 4,500	~ 4,500	~ 3,500	~ 3,000	~ 3,000	~ 3,000
盐浓度范围	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M
最高使用温度	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC	~ 80^oC
流动相兼容性	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic	Aqueous and organic

订购信息

Sepax SRT SEC-100

产品名称	粒度	孔径	规格	订货号
SRT SEC-100	5um	100 Å	21.5x600mm	215100-21560
SRT SEC-100	5um	100 Å	21.5x300mm	215100-21530
SRT SEC-100	5um	100 Å	7.5x600mm	215100-7560
SRT SEC-100	5um	100 Å	7.8x300mm	215100-7830
SRT SEC-100	5um	100 Å	7.8x150mm	215100-7815
SRT SEC-100	5um	100 Å	7.8x50 (Guard)	215100-7805
SRT SEC-100	5um	100 Å	4.6x300mm	215100-4630
SRT SEC-100	5um	100 Å	4.6x150mm	215100-4615
SRT SEC-100	5um	100 Å	4.6x50 (Guard)	215100-4605

Sepax SRT SEC-150

产品名称	粒度	孔径	规格	订货号
SRT SEC-150	5um	150 Å	21.5x600mm	215150-21560
SRT SEC-150	5um	150 Å	21.5x300mm	215150-21530
SRT SEC-150	5um	150 Å	7.5x600mm	215150-7560
SRT SEC-150	5um	150 Å	7.8x300mm	215150-7830
SRT SEC-150	5um	150 Å	7.8x150mm	215150-7815
SRT SEC-150	5um	150 Å	7.8x50 (Guard)	215150-7805
SRT SEC-150	5um	150 Å	4.6x300mm	215150-4630
SRT SEC-150	5um	150 Å	4.6x150mm	215150-4615
SRT SEC-150	5um	150 Å	4.6x50 (Guard)	215150-4605

Sepax SRT SEC-300

产品名称	粒度	孔径	规格	订货号
SRT SEC-300	5um	300 Å	21.5x600mm	215300-21560
SRT SEC-300	5um	300 Å	21.5x300mm	215300-21530
SRT SEC-300	5um	300 Å	7.5x600mm	215300-7560
SRT SEC-300	5um	300 Å	7.8x300mm	215300-7830
SRT SEC-300	5um	300 Å	7.8x150mm	215300-7815
SRT SEC-300	5um	300 Å	7.8x50 (Guard)	215300-7805
SRT SEC-300	5um	300 Å	4.6x300mm	215300-4630
SRT SEC-300	5um	300 Å	4.6x150mm	215300-4615
SRT SEC-300	5um	300 Å	4.6x50 (Guard)	215300-4605

Sepax SRT SEC-500

产品名称	粒度	孔径	规格	订货号
SRT SEC-500	5um	500 Å	21.5x600mm	215500-21560
SRT SEC-500	5um	500 Å	21.5x300mm	215500-21530
SRT SEC-500	5um	500 Å	7.5x600mm	215500-7560
SRT SEC-500	5um	500 Å	7.8x300mm	215500-7830
SRT SEC-500	5um	500 Å	7.8x150mm	215500-7815
SRT SEC-500	5um	500 Å	7.8x50 (Guard)	215500-7805
SRT SEC-500	5um	500 Å	4.6x300mm	215500-4630
SRT SEC-500	5um	500 Å	4.6x150mm	215500-4615
SRT SEC-500	5um	500 Å	4.6x50 (Guard)	215500-4605

Sepax SRT SEC-1000

产品名称	粒度	孔径	规格	订货号
SRT SEC-1000	5um	1000 Å	21.5x600mm	215950-21560
SRT SEC-1000	5um	1000 Å	21.5x300mm	215950-21530
SRT SEC-1000	5um	1000 Å	7.5x600mm	215950-7560
SRT SEC-1000	5um	1000 Å	7.8x300mm	215950-7830
SRT SEC-1000	5um	1000 Å	7.8x150mm	215950-7815
SRT SEC-1000	5um	1000 Å	7.8x50 (Guard)	215950-7805
SRT SEC-1000	5um	1000 Å	4.6x300mm	215950-4630
SRT SEC-1000	5um	1000 Å	4.6x150mm	215950-4615
SRT SEC-1000	5um	1000 Å	4.6x50 (Guard)	215950-4605

Sepax SRT SEC-2000

产品名称	粒度	孔径	规格	订货号
SRT SEC-2000	5um	2000 Å	21.5x600mm	215980-21560
SRT SEC-2000	5um	2000 Å	21.5x300mm	215980-21530
SRT SEC-2000	5um	2000 Å	7.5x600mm	215980-7560
SRT SEC-2000	5um	2000 Å	7.8x300mm	215980-7830
SRT SEC-2000	5um	2000 Å	7.8x150mm	215980-7815
SRT SEC-2000	5um	2000 Å	7.8x50 (Guard)	215980-7805
SRT SEC-2000	5um	2000 Å	4.6x300mm	215980-4630
SRT SEC-2000	5um	2000 Å	4.6x150mm	215980-4615
SRT SEC-2000	5um	2000 Å	4.6x50 (Guard)	215980-4605

注：以上为常备规格订货信息，其它规格或特殊定制产品，电询。

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SRT体积排阻色谱柱