耗材描述
产地:MBI
货号:B57
Product information
Description
The following enzymes require unique buffers for optimal digestion: AarI, AjiI, BamHI, BfuI, Bpu10I, BseXI, Bsp143I, Cfr9I, Cfr10I, Eam1105I, Ecl136II, Eco52I, EcoRI, KpnI, Lsp1109I, PacI, PasI, Ppu21I, SacI, ScaI, SdaI, SduI, SgeI and TaqI.
To ensure consistent enzyme performance, Fermentas restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.
Fermentas restriction enzymes exhibit 100% of their certified activity in the recommended buffer.
1X Composition
10 mM Tris-HCl (pH 8.0 at 37°C)
5 mM MgCl2
100 mM KCl
0.02 % Triton X-100
0.1 mg/ml BSA
Storage
All Fermentas restriction enzyme buffers should be stored at -20°C.