产地：MBI 

货号：ER0171

Conditions for 100% Activity

1X Buffer Cfr9I:

10 mM Tris-HCl (pH 7.2 at 37°C), 5 mM MgCl2, 200 mM sodium glutamate and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

Cfr9I is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 50-fold overdigestion with Cfr9I, more than 95% of the DNA fragments can be ligated and recut.