产地：MBI 

货号：ER0502

Conditions for 100% Activity

1X Buffer R:

10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

HindIII is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 50-fold overdigestion with HindIII, more than 95% of the DNA fragments can be ligated and recut.

ER0501	5000 u (10 u/µl)	1.00 ml	1.00 ml	ER0501
ER0502	5 x 5000 u (10 u/µl)	2 x 1.00 ml	1.00 ml	ER0502
ER0503	HC, 25,000 u (50 u/µl)	2 x 1.00 ml	1.00 ml	ER0503
ER0505	10000 u (10 u/µl)	4 x 1.00 ml	1.00 ml	ER0505