产地：MBI 

货号：ER0607

Conditions for 100% Activity

1X Buffer B:

10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

PaeI is supplied in:

10 mM potassium-phosphate (pH 7.0 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.5 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 50-fold overdigestion with PaeI, more than 95% of the DNA fragments can be ligated and recut.

Digestion of Agarose-embedded DNA

Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

ER0601	500 u (10 u/µl)	1.00 ml	1.00 ml	ER0601
ER0602	2500 u (10 u/µl)	1.00 ml	1.00 ml	ER0602