产地：MBI
货号：ER0612 Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
PstI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with PstI, more than 95% of the DNA fragments can be ligated and recut.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
ER0611 |
3000 u (10 u/µl) |
2 x 1.00 ml |
1.00 ml |
ER0611 |
|
ER0612 |
5 x 3000 u (10 u/µl) |
2 x 1.00 ml |
1.00 ml |
ER0612 |
|
ER0615 |
10000 u (10 u/µl) |
4 x 1.00 ml |
1.00 ml |
ER0615 |
