产地：MBI 

货号：ER0721

Conditions for 100% Activity

1X Buffer G:

10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.

Incubate at 55°C.

Storage Buffer

BclI is supplied in:

10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 10-fold overdigestion with BclI, more than 95% of the DNA fragments can be ligated and recut.