产地：MBI
货号：ER0771 Conditions for 100% Activity
1X Buffer G:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
SspI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Ligation and Recleavage
After 10-fold overdigestion with SspI, more than 90% of the DNA fragments can be ligated and recut.
ER0771 |
500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0771 |
|
ER0772 |
2500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0772 |
|
