产地：MBI 

货号：ER0821

Conditions for 100% Activity

1X Buffer B:

10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

MboII is supplied in:

10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 5-fold overdigestion with MboII, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 80% of these can be recut.

ER0821	300 u (5 u/µl)	1.00 ml	1.00 ml	ER0821
ER0822	1500 u (5 u/µl)	1.00 ml	1.00 ml	ER0822