ACADM antibody
Cat.#: 2758-1
Rabbit Monoclonal Antibody

Clone ID: EPR3707
Swiss Prot: P11310
Mol Weight: 43kDa
Size: 100ul

Description
Medium-chain acyl-CoA dehydrogenase (MCAD, ACADM) is a highly regulated member of a family of mitochondrial flavoenzymes that is essential for the beta-oxidation of medium chain fatty acids (1, 2). ACADM is active with acyl chain length of C4-C16. It is one of three similar enzymes that catalyze the initial step of fatty acid beta-oxidation (3). Deficiency of ACADM is a common inherited defect in energy metabolism (1).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 5,000
IHC: 1:250 - 500
ICC: 1:50 - 100
IP: 1:10

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

Western blot analysis on (A) fetal heart, (B) fetal liver, (C) HeLa and (D) HepG2 cell lysates using anti-ACADM RabMAb (cat. #2758-1).
Immunohistochemical analysis of paraffin-embedded human kidneytissue using anti-ACADM RabMAb (cat. #2758-1).
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACADM RabMAb (cat. #2758-1).

Specificity
A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACADM antibody, ACAD1 antibody, FLJ18227 antibody, FLJ93013 antibody, FLJ99884 antibody, MCAD antibody, MCADH antibody, Medium-chain specific acyl-CoA dehydrogenase, mitochondrial antibody, antibody

Description References
1. Zhang Z.F., et al. Biochemistry 31:81-89, 1992. 2. Kevin P. Battaile, et al. Elsevier Science B.V., 1998. 3. Kelly D.P., et al. Proc. Natl. Acad. Sci. U.S.A. 84:4068- 4072, 1987.