14-3-3 sigma(SFN)antibody
Cat.#: 5209-1
Rabbit Monoclonal Antibody
Clone ID: EPR5872
Swiss Prot: P31947
Mol Weight: 28kDa
Size: 100ul

Description
14-3-3 proteins are composed of a highly conserved multigene family of small acidic proteins. Responsive to DNA damage, 14-3-3 sigma expression is induced in a p53-dependent manner. 14-3-3 sigma sequesters cyclin B1-CDC2 complex in cytoplasm, and prevents its entry into nucleus resulting in G2/M cell cycle arrest. Furthermore, 14-3-3 sigma can link to CDK2 and CDK4 and block the cell cycle entry. Thus, 14-3-3 sigma acts as a negative regulator of cell cycle progression and is linked to cancer (1). A keratinocyte-releasable form of 14-3-3 sigma is a potent MMP-1-stimulatory factor in dermal fibroblasts. When it is released from keratinocytes, it induces the expression of MMP1, MMP3, MMP8 and MMP24 in fibroblasts through the p38 mitogen-activated protein kinase (MAPK) signaling pathway (2).

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on (A) fetal lung, (B) A549, (C) human kidney, and (D) human spleen lysates using anti-14-3-3 sigma RabMAb (cat. #5209-1).

Specificity
A synthetic peptide corresponding to residues in human 14-3-3 sigma was used as an immunogen.

Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
SFN antibody, YWHAS antibody, 14-3-3 protein sigma antibody, Epithelial cell marker protein 1 antibody, Stratifin antibody

Description References
1. Wang Z et al. BMC Cancer. 2008 Oct 24;8:308.
2. Ghaffari A et al. J Cell Sci. 2010 Sep 1;123(Pt 17):2996-3005. Epub 2010 Aug 10.