14-3-3E(YWHAE)antibody
Cat.#: 2915-1
Rabbit Monoclonal Antibody
Clone ID: EPR3919
Swiss Prot: P62258
Mol Weight: 28kDa
Size: 100ul

Description
The 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family, which consists of acidic proteins expressed at high levels in neurons of the central nervous system (1). It is an adaptor protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both insulin-like growth factor I receptor (IGFIR) and insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4).

Recommended Applications
WB, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
FC: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Products Data 

 

Western blot analysis on (A) fetal brain, (B) SW480, and (C) NIH/3T3 cell lysates using anti-14-3-3E RabMAb (cat. # 2915-1).
Immunofluorescent staining of HeLa cells using anti-14-3-3E RabMAb (cat. #2915-1).

Specificity
A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
YWHAE antibody, 14-3-3E antibody, FLJ45465 antibody, FLJ53559 antibody, KCIP-1 antibody, MDCR antibody, MDS antibody, 14-3-3 protein epsilon antibody, antibody

Description References
1. Satoh J et al. Am J Pathol. 165(2):577-92, 2004.
2. The UniProt Consortium. The Universal Protein Resource (UniPro), Nucleic Acids Res. 36:D190-D195 (2010)
3. Craparo A et al. J Biol Chem. 272(17):11663-9, 1997.
4. NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Pruitt KD, Tatusova, T, Maglott DR Nucleic Acids Res 2007 Jan 1;35(Database issue):D61-5