20S Proteasome alpha3 antibody
Cat.#: S2829
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P25788
Mol Weight: 27.65kDa
Size: 100ul

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000
IHC: 1:100-1:500

Species Reactivity
Human

Other species have not been tested

Products Data 

Sample (30ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 antibody at 1:100 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 147 of 20S Proteasome alpha3

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
PSMA3 antibody, HC8 antibody, MGC12306 antibody, MGC32631 antibody, PSC3 antibody, Proteasome subunit alpha type-3 antibody, Macropain subunit C8 antibody, Multicatalytic endopeptidase complex subunit C8 antibody, Proteasome component C8 antibody