Actopaxin/Parvin Phospho(pS8)(Parva)antibody
Cat.#: 2607-1
Rabbit Monoclonal Antibody
Clone ID: EPR2174
Swiss Prot: Q9EPC1
Mol Weight: 42kDa
Size: 100ul

Description
Actopaxin (also named Parvin, Parva) is a member of the alpha actinin superfamily containing a single alpha-actinin-like actin-binding domain. The Parvin family plays a role in cell adhesion and cytoskeleton organization. Analysis of Actopaxin and its para- and orthologues suggest, that the parvins represent a new family of alpha-actinin-related proteins that mediate cell-matrix adhesion (1). Actopaxin is a widely expressed protein that binds directly to both F-actin and paxillin LD1 and LD4 motifs. It exhibits robust focal adhesion localization in several cultured cell types, but it is not found along the length of the associated actin-rich stress fibers. Actopaxin colocalizes with paxillin to rudimentary focal complexes at the leading edge of migrating cells. Results suggest an important role for actopaxin in integrin-dependent remodeling of the actin cytoskeleton during cell motility and cell adhesion (2).

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:2500 - 5000
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data 

A. Western blot analysis on A549 cell lysates using anti- Phospho-Actopaxin (pS8) RabMAb (cat. #2607-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-Actopaxin RabMAb (cat. # 2607-1).

Specificity
A phospho specific peptide corresponding to residues surrounding serine 8 of mouse Parvin was used as an immunogen. This antibody detects Actopaxin phosphorylated on serine 8.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
Parva antibody, 2010012A22Rik antibody, 5430400F08Rik antibody, AI225929 antibody, AU042898 antibody, Actp antibody, CH-ILKBP antibody, Parvin antibody, Alpha-parvin antibody, Actopaxin antibody

Description References
1. Olski TM et al. J Cell Sci 114(Pt.3):525-38, 2001
2. Nikolopoulos SN et al. J Cell Biol 151(7) :1435-48, 2000