AIF(AIFM1)antibody
Cat.#: 1020-1
Rabbit Monoclonal Antibody
Clone ID: E20
Swiss Prot: O95831
Mol Weight: 67kDa
Size: 100ul

Description
AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000
IHC: 1:500
ICC: 1:500
IP: 1:10
FC: 1:50

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (catalog #1020-1), 1:1,000 dilution.
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (catalog #1020-1).
C. Immunofluorescent staining of HeLa cells, using AIF RabMAb. (Green fluorescence- AIF localized in mitochondria, Red - PI nuclear staining). (catalog #1020-1).
 

D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (catalog #1020-1) or a rabbit IgG (negative)(green).

Specificity
A synthetic peptide corresponding to residues near the C-terminus of Human AIF was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AIFM1 antibody, AIF antibody, COXPD6 antibody, MGC111425 antibody, PDCD8 antibody, Apoptosis-inducing factor 1, mitochondrial antibody, Programmed cell death protein 8 antibody

Description References
1. Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 441
2. Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 118
3. Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):729
4. Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 571

Product References
1. Cao GD, Xing J, Xiao X, et al, Critical Role of Calpain I in Mitochondrial Release of Apoptosis-Inducing Factor in Ischemic Neuronal Injury ,The Journal of Neuroscience 27 (2007) 9278 –9293
[Application: WB]
2. Ishimura R, Martin GR and Ackerman SL, Loss of Apoptosis-Inducing Factor Results in Cell-Type-Specific Neurogenesis Defects ,The Journal of Neuroscience 28 (2008) 4938–4948
[Application: IHC]
3. Bajt ML, Farhood A, Lemasters JJ, et al, Mitochondrial Bax Translocation Accelerates DNA Fragmentation and Cell Necrosis in a Murine Model of Acetaminophen Hepatotoxicity ,JPET 324 (2008) 8–14
[Application: WB]
4. Bajt ML, Cover C, Lemasters JJ, et al, Nuclear Translocation of Endonuclease G and Apoptosis-Inducing Factor during Acetaminophen-Induced Liver Cell Injury ,Toxicologial Sciences 94 (2006) 217–225
[Application: IHC]
5. Yu SW, Andrabi SA, Wang HM, et al, Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death ,PNAS 103 (2006) 18314 –18319
[Application: ICC]
6. Lin CY, Wu HY, Wang PL, et al, Mammalian Ste20-like protein kinase 3 induces a caspase-independent apoptotic pathway ,Int J Biochem Cell Biol 42 (2010) 98-105
[Application: WB]
7. Shen H, Hu X, Liu C, et al, Ethyl pyruvate protects against hypoxic-ischemic brain injury via anti-cell death and anti-inflammatory mechanisms ,Neurobiol Dis 37 (2010) 711-722
[Application: WB]
8. Chen X, Gong J, Zeng H, et al, MicroRNA145 Targets BNIP3 and Suppresses Prostate Cancer Progression ,Cancer Res 70 (2010) 2728-2738
9. Saito C, Lemasters JJ, Jaeschke H, c-Jun N-terminal kinase modulates oxidant stress and peroxynitrite formation independent of inducible nitric oxide synthase in acetaminophen hepatotoxicity ,Toxicol Appl Pharmacol 246 (2010) 8-17
[Application: WB]
10. Huang R, Chen XQ, Huang Y, et al, The multikinase inhibitor sorafenib induces caspase-dependent apoptosis in PC-3 prostate cancer cells ,Asian J Androl 12 (2010) 527-534
[Application: WB]