Aldh2(ALDH2)antibody
Cat.#: 3221-1
Rabbit Monoclonal Antibody
Clone ID: EPR4493
Swiss Prot: P05091
Mol Weight: 53kDa
Size: 100ul

Description
Aldh2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. Aldh2 encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix (1).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) HepG2 and (B) A549 cell lysates using anti-Aldh2 RabMAb (cat. #3221-1).
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Aldh2 RabMAb (cat. #3221-1).
Western blot analysis on (A) mouse liver and (B) rat liver lysates using anti-Aldh2 RabMAb (cat. #3221-1).

Specificity
A synthetic peptide corresponding to residues in human Aldh2 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ALDH2 antibody, ALDH-E2 antibody, ALDHI antibody, ALDM antibody, MGC1806 antibody, Aldehyde dehydrogenase, mitochondrial antibody, ALDH class 2 antibody, ALDH-E2 antibody, ALDHI antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.