Recombinant Human Creatine Kinase Brain, His Tag
人肌酸激酶  (CKB His)
Catalogue Number  CKI-274

Synonyms:
EC 2.7.3.2, Creatine kinase B chain, Creatine kinase B type, CKB, CKBBB, B-CK, Creatine Kinase Brain. 

Introduction:
CKB is a cytoplasmic enzyme that takes part in energy homeostasis. CKB enzyme reversibly catalyzes the transfer of phosphate among ATP and various phosphogens such as creatine phosphate. CKB functions as a homodimer in the brain as well as in different tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases supply the energy of phosphate hydrolysis essential to drive the normal role of many cellular systems including muscle, tumor and cancer cells. 

Description:
CKB Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 401 amino acids (1-381 a.a.) and having a molecular mass of 44.8 kDa. The CKB is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. 

Source:
Escherichia Coli. 

Physical Appearance:
Sterile filtered colorless solution. 

Formulation:
CKB Human solution containing 20mM Trsi pH-8, 1mM DTT, & 10% glycerol. 

Stability:
CKB Human although stable at 4°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles. 

Purity:
Greater than 95.0% as determined by SDS-PAGE. 

Amino acid sequence:
MGSSHHHHHH SSGLVPRGSH MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD
RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K. 

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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CKB His 人肌酸激酶 CKI-274 Recombinant Human Creatine Kinase Brain, His Tag
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