Apolipoprotein J(CLU)antibody
Cat.#: 2709-1
Rabbit Monoclonal Antibody
Clone ID: EPR2911
Swiss Prot: P10909
Mol Weight: 70kDa
Size: 100ul

Description
Apolipoprotein J (ApoJ) is a 70-kD protein associated with high-density lipoproteins (HDL) in human plasma. The protein is synthesized as a 427-amino acid polypeptide that is posttranslationally cleaved at an internal bond between arg205 and ser206. Two subunits, designated alpha (34 to 36 kD), corresponding to residues 1-205, and beta (36 to 39 kD), corresponding to residues 206-427, are associated through disulfide bonds (1). ApoJ is a stress-induced cytoprotective chaperone protein, regulated by HSF1, and functions similarly to a small heat-shock protein. ApoJ is expressed in a variety of cancers and associated with broad-spectrum treatment resistance (2).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:1000 - 2000
IHC: 1:100 - 250

Species Reactivity
Human

Products Data 

 

Western blot analysis on (A) Human tonsil tissue (B) fetal brain and (C) human plasma lysate using anti-ApoJ RabMAb (cat. #2709-1).
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ApoJ RabMAb (cat. #2709-1).

Specificity
A synthetic peptide corresponding to residues in the alpha chain of human ApoJ was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CLU antibody, APOJ antibody, CLI antibody, KUB1 antibody, MGC24903 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, Clusterin alpha chain antibody, ApoJbeta antibody, Complement cytolysis inhibitor b chain antibody

Description References
1. de Silva HV, et al. J Biol Chem 265 :14292-14297, 1990 2. Chi KN, et al. Expert Opin Investig Drugs 17(12) :1955-62, 2008