actinin, alpha 2 antibody
Cat.#: S2341
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P35609
Mol Weight: 103.87kDa
Size: 100ug

Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000,
IF: 1:100-1:200
ICC: 1:100-1:200

Species Reactivity
Human

Other species have not been tested

Products Data 

 

Sample (30ug of whole cell lysate)
A: A431
B: H1299
7.5% SDS PAGE
diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2 antibody at 1:200 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 215 and 500 of actinin, alpha 2

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
ACTN2 antibody, CMD1AA antibody, Alpha-actinin-2 antibody, Alpha-actinin skeletal muscle isoform 2 antibody, F-actin cross-linking protein antibody