| Catalogue Number | ANT-148 |
| Synonyms | CD8, MAL, p32. |
| Type | Mouse Anti Human Monoclonal. |
| Introduction | CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 acts as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional CD8 is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
CD8 identifies cytotoxic/suppressor t-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of t-cell mediated killing. CD8 alpha chains binds to class-I MHC molecules alpha-3 domains. |
| Clone | hCD8. |
| Immunogen | Purified human PBL CD8+ T cells. |
| Ig Subclass | Mouse IgG2a. |
| Purification Method | Ion exchange column. |
| Protein concentration | 1mg/ml in PBS (after reconstitution). |
| Storage Procedures | Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. |
| Reconstitution | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC. |
| Antibody Properties | Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking T cell activation should be determined by the investigator. |
| Available Conjugates | This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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