| Catalogue Number | ANT-250 |
| Synonyms | Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta. |
| Type | Mouse Anti Human Monoclonal. |
| Introduction | Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Clone | NYR-hIL1?. |
| Immunogen | r.Human IL-1b. |
| Ig Subclass | Mouse IgG2b. |
| Purification Method | Ion Exchange. |
| Protein concentration | 1 mg/ml in PBS (after reconstitution). |
| Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. |
| Storage Procedures | In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
| Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. |
| Patent Rights | The sale and/or commercial use of Recombinant Human IL1B is prohibited in the United States of America (U.S.A) and Japan. |
| Applications | Western Blot, immunohistochemistry intra-cellular staining and will also work in Direct ELISA and Immuneprecipitation. |
| Titer | For intra-cellular staining, 10µl per 106 cells. By direct ELISA, 1:10,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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