Apolipoprotein E(N-term)(APOE)antibody
Cat.#: 1981-1
Rabbit Monoclonal Antibody
Clone ID: EP1373Y
Swiss Prot: P02649
Mol Weight: 36kDa
Size: 100ul

Description
The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism. In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol. Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein (1). Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease (2). Three major isoforms of Apo E (E2, E3, E4) has been identified, with E3 being the most common in human. E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease (3).

Recommended Applications
WB, IHC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:500 - 1000
IHC: 1:100 - 1:250
IP: 1:70
FC: 1:30

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on human serum using anti-Apo E RabMAb. (Cat. # 1981-1).
B. Immunohistochemical analysis of paraffin-embedded human fetal liver using anti-Apo E RabMAb (cat. # 1981-1).

Specificity
A synthetic peptide corresponding to residues near the N-term of human apolipoprotein E was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APOE antibody, AD2 antibody, LDLCQ5 antibody, LPG antibody, MGC1571 antibody, Apolipoprotein E antibody, antibody

Description References
1. Zaioua M et al, Journal of Lipid Research, 41:1087-1095, 2000
2. Mahley RW and SC Rall, Jr. 7th ed. McGraw-Hill 1995
3. Olarte L et al, Arch Neurol. 63(11):1586-90, 2006