ADRA2B Antibody(Center)
Cat.#: T3257
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P18089
Mol Weight: 50kDa
Size: 100ug

Description
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.

Recommended Applications
WB, IHC, FC, ELISA

Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of ADRA2B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ADRA2B (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ADRA2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349~378 amino acids from the Center region of human ADRA2B.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Alternative Names
ADRA2B antibody, ADRA2L1 antibody, ADRA2RL1 antibody, ADRARL1 antibody, ALPHA2BAR antibody, Alpha-2B adrenergic receptor antibody, Alpha-2 adrenergic receptor subtype C2 antibody, Alpha-2B adrenoreceptor antibody

Description References
Tabakoff,B., et.al., BMC Biol. 7, 70 (2009)
Weinshank,R.L., et.al. Mol. Pharmacol. 38 (5), 681-688 (1990)