AP2S1 antibody
Cat.#: 2647-1
Rabbit Monoclonal Antibody
Clone ID: EPR2696
Swiss Prot: P53680
Mol Weight: 17kDa
Size: 100ul

Description
Adaptor-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles. It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1). AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (1, 2).

Recommended Applications
WB, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 5,000
ICC: 1:100 - 250
IP: 1:10
FC: 1:200

Species Reactivity
Human, Mouse, Rat

Species reactivity based on WB testing only

Products Data

A. Western blot analysis on (A) fetal brain and (B) HeLa cell lysates using anti-AP2S1 RabMAb (cat. #2647-1).

Specificity
A synthetic peptide corresponding to residues in human AP2S1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AP2S1 antibody, AP17 antibody, AP17-DELTA antibody, CLAPS2 antibody, AP-2 complex subunit sigma antibody, Adapter-related protein complex 2 sigma subunit antibody, Adaptor protein complex AP-2 subunit sigma antibody, Clathrin assembly protein 2 small chain antibody, Clathrin coat assembly protein AP17 antibody, Clathrin coat-associated protein AP17 antibody, HA2 17 kDa subunit antibody, Plasma membrane adaptor AP-2 17 kDa protein antibody, Sigma2-adaptin antibody

Description References
1. Kirchhausen T, et al. J Biol Chem. 266(17):11153-7, 1991 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)