Aldh1A1(ALDH1A1)antibody
Cat.#: 2052-1
Rabbit Monoclonal Antibody
Clone ID: EP1933Y
Swiss Prot: P00352
Mol Weight: 55kDa
Size: 100ul

Description
Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:30

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on HepG2 cell lysate using anti-Aldh1a1 RabMAb (cat. # 2052-1), dilution 1:2000.
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-Aldh1a1 RabMAb (cat. #2052-1).

Specificity
A synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ALDH1A1 antibody, ALDC antibody, ALDH-E1 antibody, ALDH1 antibody, ALDH11 antibody, MGC2318 antibody, PUMB1 antibody, RALDH1 antibody, Retinal dehydrogenase 1 antibody, ALDH-E1 antibody, ALHDII antibody, Aldehyde dehydrogenase family 1 member A1 antibody, Aldehyde dehydrogenase, cytosolic antibody

Description References
1. Yoshida A, et al. Alcohol 2(1):103-6, 1985
2. Agarwal DP, et al. Enzyme 42(1):47-52, 1989
3. Collard F, et al. Biochimie 89(3):369-73, 2007